Coding

Part:BBa_K4214003:Design

Designed by: Jol Tekoniemi   Group: iGEM22_Stockholm   (2022-10-08)


hGIF with LacI promotor and 6xHis-tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 485
    Illegal BsaI.rc site found at 1165
    Illegal SapI site found at 1155


Design Notes

The two parts were cloned together using restriction digestion.


Source

The human GIF comes from the human genome, chromosome 11q12.1. The lacI sequence originates from

References